apo1 fas  (Novus Biologicals)

Journal: Cell

Article Title: Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis

doi: 10.1016/j.cell.2021.04.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-FAS/Apo1/CD95 , Biovision , Cat# 2070R.

Techniques: Virus, Recombinant, Western Blot, Giemsa Stain, Protease Inhibitor, Caspase-Glo Assay, Plasmid Preparation, Labeling, In Situ, Proximity Ligation Assay, Transfection, Magnetic Beads, Software, Cell Culture, Membrane

Journal: DNA and Cell Biology

Article Title: KHDC1A, a Novel Translational Repressor, Induces Endoplasmic Reticulum-Dependent Apoptosis

doi: 10.1089/dna.2012.1682

Figure Lengend Snippet: ER is involved in KHDC1A-induced apoptosis. (A) Overexpressed KHDC1A is colocalized with ER. HeLa cells were transfected with KHDC1A or KHDC1B. Immunostaining was performed to determine the expression of KHDC1A, KHDC1B (FITC, green), and ER marker Calnexin (Cy5, red). Scale bar was 16 μm. (B) C-terminus of KHDC1A determines the ER localization. HeLa cells were transfected with Flag-tagged KHDC1A-tr or KHDC1B-fu. Immunostaining was performed to determine the expression of KHDC1A-tr, KHDC1B-fu (FITC, green), and ER marker Calnexin (Cy5, red). Scale bar was 16 μm. (C) The cleavage of caspase-12 is detected in Flag-tagged KHDC1A-transfected Hela cells. HeLa cells were transfected with control vector, Flag-tagged KHDC1A, or KHDC1B plasmids. The cells were harvested, and Western blot analysis was performed with antibodies against Flag, caspase-12, cleaved PARP, and β-tubulin. (D) Caspase-12 specific inhibitor Z-ATAD rescues KHDC1A-induced apoptosis. HeLa cells were transfected with KHDC1A. Transfected cells were treated with 20 μM Z-ATAD, Z-DEVD, or DMSO as control. Twenty-four hours after transfection, the cells were harvested, and apoptosis was assayed by flow cytometry. Data were summarized from three independent experiments. (E) Caspase-12 specific inhibitor Z-ATAD cannot rescue Fas-induced apoptosis. Hela cells were pretreated with mock or 1 μg/mL of anti-Fas/Apo1 antibodies (Biovision) for 6 h and then exposed to 20 μM of caspase-12 inhibitors Z-ATAD FMK for another 48 h. Cells were then harvested, and apoptosis was assayed by flow cytometry. Data were summarized from three independent experiments. ER, endoplasmic reticulum.

Article Snippet: Hela cells were pretreated with mock or 1 μg/mL of anti-Fas/Apo1 antibodies (Biovision) for 6 h and then exposed to 20 μM of caspase-12 inhibitors Z-ATAD FMK for another 48 h. Cells were then harvested, and apoptosis was assayed by flow cytometry.

Techniques: Transfection, Immunostaining, Expressing, Marker, Plasmid Preparation, Western Blot, Flow Cytometry